Institut Pasteur

About Institut Pasteur

The Single Cell Biomarkers Unite for Technology and Services (scBiomarkers UTechS) is affiliated with the Center for Technological Resources and Research (C2RT) and the Immunology Department of the Institut Pasteur. The mission of the scBiomarkers UTechS is to facilitate biomedical and fundamental research through high-tech equipment for functional cell assays, multi-color phenotyping, cell sorting, profiling of proteins and transcripts, and single-cell multi-OMICS. One of the scBiomarker’s strengths is its Bsl2/Bsl2+ laboratories for handling human and infectious material. We provide project-tailored support, spanning from experimental design, and sample processing, to data analysis. Our current technological portfolio includes 25 cutting-edge technologies installed in 6 dedicated laboratories. Our expertise covers: Cell culture and functional cell assays under controlled gas composition (in Bsl2+ environment), Cell phenotyping and cell sorting by conventional, spectral, and imaging flow cytometry, image- and fluorescence-based single-cell sorting, Live-content imaging, Protein profiling by multiplex, ultrasensitive, single-cell immunoassays, RNA/DNA profiling, Single-cell multi-OMICS and spatial transcriptomicsBioinformatics, Data analysis (sc RNA-Seq, multidimensional flow cytometry), Advanced immunomonitoring. A significant part of the equipment is situated within two fully equipped Bsl2+ cell culture laboratories allowing manipulation of human and infectious material. Since 2011 the scBiomarkers UTechS has been ISO9001 certified, providing quality assurance of our service. We are an IBISA-labeled platform as well as an active member and Center of Excellence of the Federation of Clinical Immunology Laboratories (FOCIS). Our ongoing developments focus on various novel pipelines for single-cell multi-OMICS analysis, and on studies of immune profiles for the discovery of biomarkers in vaccinology and in different pathological contexts.